Anti-CAMLG Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49069

Core Product Specifications and Parameters

Biological Background: CAMLG Function and Localization

• CAMLG, also known as CAML, GET2, or CDG2Z, is a crucial component of the guided entry of tail-anchored (TA) protein pathway.
• Functions as a membrane receptor for the GET3/TRC40 complex, facilitating the post-translational insertion of TA proteins into the endoplasmic reticulum (ER) membrane. Related references: PMID:23041287 | PMID:24392163 | PMID:27226539
• By interacting with GET1/WRB, it stabilizes GET1 and mediates the delivery of TA proteins, a process essential for maintaining cellular protein homeostasis. Related references: PMID:32187542
• Stimulates calcium signaling in T cells by elevating intracellular calcium levels, linking protein trafficking to immune cell activation. Related references: PMID:7522304
• Subcellularly localized to the endoplasmic reticulum membrane, consistent with its role in TA protein capture and insertion.
• Ubiquitously expressed with highest levels in brain, testis, and ovary, suggesting specialized functions in neuroendocrine and reproductive tissues.
• Post-translational modifications include disulfide bond formation and phosphorylation (as indicated by UniProt keywords).
• Associated with congenital disorder of glycosylation and host-virus interactions, highlighting its broader biomedical relevance.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 1–190 of human CAMLG (NP_001736.1), covering the N-terminal cytosolic domain; consider that this antibody may recognize epitopes within this region in both denaturing and native conditions.
• For Western blot, a band near the predicted molecular weight of 33 kDa is expected, though post-translational modifications or dimerization could affect migration; validate in your sample system.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), ER localization should be observed; co-staining with ER markers can help confirm specificity.
• Cross-reactivity with mouse and rat orthologs is reported; sequence alignment suggests conserved epitopes, but validation in the target species is recommended.
• The antibody is applicable in ELISA; optimal coating concentrations and detection conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions. With this anti-CAMLG polyclonal antibody, researchers and diagnostic developers studying tail-anchored protein trafficking, ER biology, or calcium signaling can access reliable immunodetection tools. For customized assay development or large-scale supply needs, our technical team provides end-to-end support from concept to clinic.