Anti-CAMKK2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96RR4

Core Product Specifications and Parameters

Biological Background: CAMKK2 Function and Localization

• CAMKK2 (Calcium/calmodulin-dependent protein kinase kinase 2), also known as CaM-KK beta, is a serine/threonine kinase that belongs to a proposed calcium-triggered signaling cascade.
• It phosphorylates and activates downstream kinases including CAMK1, CAMK4, and AMPK, thereby regulating various cellular processes (By similarity).
• Multiple isoforms exist due to alternative splicing; isoform 1 and 2 phosphorylate CAMK1 and CAMK4, isoform 3 additionally targets CAMK1D, while isoforms 4–6 are inactive due to partial calmodulin-binding domain loss.
• The protein is involved in hippocampal activation of CREB1 and may play roles in neurite growth, with isoform 3 promoting elongation and isoform 1 promoting branching (By similarity).
• Subcellularly, CAMKK2 is found in the nucleus, cytoplasm, and neuronal projections.
• It is ubiquitously expressed with highest levels in the brain, and intermediate levels in spleen, prostate, thyroid and leukocytes; lowest in lung.
• Key structural features include an ATP-binding domain, calmodulin-binding domain, and multiple phosphorylation and acetylation sites.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–100) targets the N‑terminal region of human CAMKK2; consider that epitope accessibility may vary across applications and isoforms.
• In WB, a band around 65 kDa is expected; additional bands from isoforms or post‑translational modifications may appear, so validate in relevant sample systems with proper controls.
• For IF/ICC, optimize fixation and permeabilization conditions to detect nuclear, cytoplasmic, and neuronal projection pools.
• In ELISA, titrate the antibody to achieve optimal signal‑to‑noise; pairing with a matched detection antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one‑stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This CAMKK2 polyclonal antibody enables investigations into calcium signaling, neuronal function, and kinase cascades—areas where we also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Rely on our integrated supply capabilities to accelerate your assay development and biomarker validation workflows.