Anti-CAMKK1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8N5S9

Core Product Specifications and Parameters

Biological Background: CAMKK1 Function and Localization

• Calcium/calmodulin-dependent protein kinase kinase 1 (CAMKK1, also known as CaM-KK alpha) phosphorylates and activates downstream kinases CAMK1, CAMK1D, CAMK1G, and CAMK4 in a calcium-triggered signaling cascade.
• CAMKK1 plays a role in regulating apoptosis and cell survival by phosphorylating AKT1/PKB, which inhibits the pro-apoptotic protein BAD.
• The protein is localized to both the cytoplasm and nucleus.
• Alternative splicing produces multiple isoforms.
• Post-translational modifications include phosphorylation and methylation.
• CAMKK1 is a serine/threonine-protein kinase that binds calmodulin and ATP.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 399-520) targets a region outside the kinase domain, which may help detect multiple CAMKK1 isoforms or avoid cross-reactivity with other CAMKK family members; however, verify specificity with knockout controls.
• For Western blot, a specific band at approximately 56 kDa is expected in human and mouse cell lysates; consider using whole-cell extracts from cell lines known to express CAMKK1 (e.g., HeLa, NIH/3T3).
• In immunohistochemistry (paraffin-embedded), optimize antigen retrieval and dilution; nuclear and cytoplasmic staining is predicted based on subcellular localization.
• ELISA applications may be suitable for quantitative detection; pair with an appropriate capture or detection antibody and validate with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by providing comprehensive access to raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. For calcium signaling and kinase research, CamelBio offers reliable CAMKK1 antibodies and can assist in sourcing rare targets and custom reagents to accelerate your assay development.