Anti-CaMKII Rabbit Polyclonal Antibody for WB and ELISA - Q13554

Core Product Specifications and Parameters

Biological Background: CaMKIIβ (CAMK2B) Function and Localization

• CAMK2B encodes the beta subunit of Ca2+/calmodulin-dependent protein kinase II, a multifunctional serine/threonine kinase involved in key cellular processes.
• Plays a dual role in neurons: kinase-dependent signaling and kinase-independent actin bundling, crucial for dendritic spine and synapse formation, long-term potentiation (LTP), and hippocampus-dependent learning. Related references: PMID:29100089
• In skeletal muscle, modulates sarcoplasmic reticulum Ca2+ transport: in slow-twitch muscle regulates Ca2+ uptake; in fast-twitch muscle controls Ca2+ release via phosphorylation of triadin and phospholamban. Related references: PMID:16690701
• Under endoplasmic reticulum stress, phosphorylates reticulophagy regulator RETREG1 at Ser-151, enhancing its oligomerization and reticulophagy activity. Related references: PMID:31930741
• Localizes to cytosol, cytoskeleton, centrosome, sarcoplasmic reticulum membrane, and synapse, reflecting its diverse functional sites.
• Widely expressed, with notable presence in adult and fetal brain and skeletal muscle, consistent with roles in neurodevelopment and muscle physiology.
• Functional keywords include alternative splicing, actin-binding, calmodulin-binding, developmental protein, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to N-terminal amino acids 1-300 of human CaMKII beta (NP_742078.1). This region may contain conserved epitopes, consistent with cross-reactivity to mouse and rat.
• The recombinant protein immunogen suggests suitability for ELISA applications; consider optimizing coating and detection conditions for your specific assay format.
• For western blotting, expect a band at approximately 73 kDa. Validate in relevant sample systems (e.g., brain or skeletal muscle lysates) and include appropriate loading and molecular weight controls.
• CaMKII beta exerts both kinase-dependent and structural functions; total protein levels may not directly correlate with activity. Activity assays or phospho-specific antibodies may complement expression analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting — covering every stage from concept to clinic. For CaMKIIβ‑related neuroscience and muscle signaling research, our supply chain includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your project.