Anti-CaMKII Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q13554

Core Product Specifications and Parameters

Biological Background: CaMKII beta Function and Localization

• CaMKII beta (also known as CAM2, CAMK2, CAMKB, CaMKIIβ) is a serine/threonine-protein kinase encoded by the CAMK2B gene.
• This multifunctional kinase becomes autonomous after Ca2+/calmodulin binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity, and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. Related references: PMID:16690701
• In neurons, CaMKII beta has a kinase-independent structural role, binding and bundling actin filaments to reorganize the actin cytoskeleton, which is critical for dendritic arborization and remodeling. Related references: PMID:29100089
• Under endoplasmic reticulum stress, CaMKII beta phosphorylates the reticulophagy regulator RETREG1 at Ser-151, enhancing its oligomerization, membrane scission, and reticulophagy activity. Related references: PMID:31930741
• The protein localizes to multiple subcellular compartments, including the cytoplasm, cytoskeleton, centrosome, sarcoplasmic reticulum membrane, and synapse.
• CaMKII beta is widely expressed, with high levels in adult and fetal brain and skeletal muscle, consistent with its neurological and muscular roles.
• Contains conserved ATP-binding and calmodulin-binding domains characteristic of CaMK family serine/threonine kinases.

Experimental Guidance and Technical Tips

• The immunogen consists of a synthetic peptide spanning amino acids 550–650 of human CaMKII beta; consider potential isoform-specific or post-cleavage detection in your assay.
• For Western blotting, validate specificity using appropriate controls, as the predicted molecular weight is ~73 kDa, but post-translational modifications may cause shifts.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody titrations; include positive and negative tissue controls.
• For ELISA applications, ensure suitable coating buffers and detection conditions are empirically determined for your target analyte.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies like this CaMKII Rabbit mAb, as well as bulk ancillary reagents and custom sourcing for rare targets. Whether you are developing assays for neurodegenerative diseases, muscle biology, or kinase signaling, CamelBio is your reliable partner for scaling up production and ensuring consistent lot-to-lot performance.