Anti-CaMKII Rabbit Monoclonal Antibody for WB and ELISA - Q9UQM7/Q13554/Q13557/Q13555

Core Product Specifications and Parameters

Biological Background: CaMKII Function and Localization

• Calcium/calmodulin-dependent protein kinase type II (CaMKII) is encoded by four genes (CAMK2A, CAMK2B, CAMK2D, CAMK2G) giving rise to multiple isoforms with distinct molecular weights (54–72 kDa).
• Functions as a key synaptic plasticity regulator by mediating Ca²⁺/calmodulin-dependent autophosphorylation, which enables autonomous kinase activity and contributes to long-term potentiation. Related references: PMID:14722083
• Regulates NMDAR-dependent AMPAR potentiation at excitatory synapses, and is essential for dendritic spine development. Related references: PMID:28130356
• Involved in neuronal migration during development, and phosphorylates transcription factors FOXO3 and STAT1, linking it to gene expression control and immune signaling. Related references: PMID:29100089 PMID:23805378 PMID:11972023
• Mediates interferon-beta signaling through JAK-STAT pathway activation and modulates ferroptosis via PSAT1 phosphorylation. Related references: PMID:35568036 PMID:40281343
• Localizes to the postsynaptic density, dendritic spines, and dendrites, consistent with its role in synaptic signal transduction.
• Keywords: ATP-binding, calmodulin-binding, kinase, lipoprotein, palmitate, phosphoprotein, serine/threonine-protein kinase, synapse.

Experimental Guidance and Technical Tips

• For Western blotting, consider extracting protein from brain tissue or neuronal cell lines (human, mouse, or rat) and validate detection of multiple isoforms at 54–72 kDa.
• Due to the monoclonal nature of this antibody, expect consistent recognition of the immunogen region across experiments; pre-test on positive control lysates is advised.
• In ELISA applications, direct or sandwich formats can be explored using recombinant CaMKII protein or synthetic peptide; optimize coating conditions and antibody concentration.
• Given the cross-reactivity with rodent species, this antibody may be suitable for comparative studies; perform validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined supply channel for IVD raw materials, bridging the gap between concept and clinic. This CaMKII monoclonal antibody supports neuroscience and signal transduction research, and through our network we can provide complementary validated antibody pairs, bulk ancillary reagents, and assistance in sourcing rare-target raw materials. Let us help accelerate your diagnostic development with reliable bioproducts.