Anti-CaMKII delta Polyclonal Antibody for WB, ELISA - Q13557

Core Product Specifications and Parameters

Biological Background: CaMKII delta Function and Localization

• CaMKII delta (CAMKD, CAMK2D) is a calcium/calmodulin-dependent protein kinase that plays a critical role in cardiac excitation-contraction coupling by regulating Ca2+ homeostasis through phosphorylation of key substrates such as RYR2, PLN, and CACNB2.
• In the nucleus, it phosphorylates the MEF2 repressor HDAC4, leading to its nuclear export and activation of the hypertrophic gene program (PubMed:17179159).
  Related references:
  PMID:17179159
• It phosphorylates phospholamban (PLN) to enhance sarcoplasmic reticulum Ca2+ uptake, important for frequency-dependent acceleration of relaxation and contractile function during acidosis (PubMed:16690701).
  Related references:
  PMID:16690701
• Located at the sarcolemma and sarcoplasmic reticulum membrane, CaMKII delta directly interacts with and modulates ion channels such as Nav1.5/SCN5A and Kv4.3/KCND3, impacting cardiac arrhythmogenesis.
• Multiple alternative splicing isoforms (e.g., delta 2, 3, 8, 9 in heart and delta 11 in skeletal muscle) exhibit tissue-specific expression and functional specialization.
• Classified as a serine/threonine kinase and calmodulin-binding protein, CaMKII delta is involved in ATP binding and phosphoprotein signaling, with implications in dilated cardiomyopathy and heart failure.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-260, encompassing a region within the N-terminal kinase domain, which may allow recognition of multiple CaMKII delta isoforms; consider validating specificity in tissue-specific lysates.
• For Western blot, utilize cardiac or skeletal muscle homogenates (mouse and human) due to high expression; a band at ~56 kDa is expected, though alternative isoforms may produce additional bands.
• When performing ELISA, pairing with a CaMKII delta-specific detection antibody is recommended; consider cross-reactivity with mouse samples as indicated.
• Include appropriate controls such as antigen pre-incubation or CRISPR knockout samples to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your cardiac and skeletal muscle research. Our one-stop platform bridges the gap from concept to clinic, simplifying procurement for diagnostic manufacturers and research labs. For studying calcium signaling and cardiomyopathy targets like CaMKII delta, we provide tailored sourcing solutions to accelerate your assay development.