Anti-CaMKII delta (CAMKD) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q13557

Core Product Specifications and Parameters

Biological Background: CaMKII delta Function and Localization

• CaMKII delta is a calcium/calmodulin-dependent serine/threonine kinase belonging to the CAMK2 family, encoded by the CAMK2D gene; multiple splice isoforms exist, with isoform Delta 3, 2, 8, 9 expressed in cardiac muscle and Delta 11 in skeletal muscle.
• Serves as a central regulator of cardiac excitation-contraction coupling by targeting ion channels and transporters, including L-type calcium channel CACNB2, sodium channel Nav1.5/SCN5A, and potassium channel Kv4.3/KCND3.
• Mediates SR Ca2+ release by direct phosphorylation of ryanodine receptor RYR2 at Ser-2808, contributing to cardiac decompensation in heart failure.
• Phosphorylates phospholamban (PLN), relieving inhibition of SERCA2A and enhancing SR Ca2+ uptake, which is important for frequency-dependent acceleration of relaxation and contractile function during acidosis. Related references: PMID:16690701
• In the nucleus, phosphorylates HDAC4, promoting its nuclear export and enabling MEF2-driven hypertrophic gene expression. Related references: PMID:17179159
• Subcellularly localized to the sarcolemma and sarcoplasmic reticulum membrane, where it forms macromolecular complexes with its substrates.
• In response to interferon-gamma, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT pathway.
• Post-translationally regulated by acetylation and autophosphorylation; structural studies, ATP-binding, and calmodulin-binding are key functional features.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human CaMKII delta; consider using this N-terminal region to differentiate from other CAMK2 family members if specificity is critical.
• For Western blot, the predicted molecular weight is ~56 kDa; validate band patterns in cardiac tissue lysates, and include phosphatase inhibitors to preserve phosphorylation states.
• In immunofluorescence/ICC, anticipate sarcolemmal and sarcoplasmic reticulum staining; appropriate fixation and permeabilization are essential for membrane-bound targets.
• ELISA performance may be improved by using peptide immunogen as a positive control to confirm antibody affinity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with comprehensive access to IVD raw materials, including validated monoclonal antibodies for cardiac signaling targets like CaMKII delta. We offer one-stop sourcing solutions—from antibody pairs and bulk ancillary reagents to tailored technical consultation—aimed at accelerating your cardiac biomarker and heart failure assay development from concept to clinic.