Anti-CaMKI Rabbit Monoclonal Antibody for WB and ELISA - Q14012

Core Product Specifications and Parameters

Biological Background: CaMKI/CaMKI-alpha Function and Localization

• Calcium/calmodulin-dependent protein kinase type 1 (CaMKI, also known as CaM kinase I alpha) is a serine/threonine-protein kinase that belongs to the CaMK family. It functions downstream of CaMKK in the calcium-triggered signaling cascade.
• CaMKI is a key regulator of neuronal development and function. It promotes axonal extension, growth cone motility, dendritic growth, and spine/synapse formation in hippocampal neurons. It is essential for long-term potentiation (LTP) and ERK2-dependent translational activation at synapses.
• Beyond the nervous system, CaMKI regulates diverse processes including cell cycle progression (G1/S transition via cyclin-D1/CDK4), muscle cell differentiation (via HDAC5 phosphorylation and nuclear export), and hormone production (aldosterone synthase regulation in adrenal cortex).
• The kinase phosphorylates numerous substrates such as CREB1, ATF1, CFTR, MYL9, SYN1, EIF4G3, ARHGEF7, MARK2, and NUMB, thereby controlling transcription, endocytosis, and cytoskeletal dynamics.
• CaMKI is localized to both the cytoplasm and nucleus, consistent with its roles in signal transduction and transcriptional regulation.
• The protein is widely expressed across tissues, with notable expression in the adrenal cortex.
• Post-translational modifications include phosphorylation for activation and ubiquitination; it is an allosteric enzyme that binds calmodulin in a calcium-dependent manner.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100), which may be conserved across species and is likely to detect the native conformation of CaMKI. Consider pre-adsorption with the immunizing peptide to confirm specificity.
• For Western blotting, validate the antibody in lysates from human, mouse, or rat tissues/cell lines where CaMKI is expressed (e.g., brain, adrenal gland). The predicted molecular weight is approximately 41 kDa; ensure optimal gel electrophoresis and transfer conditions.
• For ELISA, the antibody can be used as a capture or detection reagent. Perform cross-titration with antigen to determine optimal working concentrations. Include appropriate positive and negative controls.
• Since CaMKI is activated by calcium/calmodulin, stimulation of cells with agents that raise intracellular Ca2+ may enhance detection in functional assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized procurement of rare targets. For research on calcium signaling and neuronal biology, CamelBio offers reliable access to CAMK1 reagents such as this rabbit monoclonal antibody. Our services support diagnostic manufacturers and research laboratories with efficient supply chains and technical consultation.