Anti-CAMK4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16566

Core Product Specifications and Parameters

Biological Background: CAMK4 Function and Localization

• Calcium/calmodulin-dependent serine/threonine-protein kinase that phosphorylates transcription activators such as CREB1, MEF2D, JUN, and RORA, playing key roles in immune response, inflammation, and memory consolidation.
• Operates in the CaMKK-CaMK4 signaling cascade and can activate MAP kinases (MAPK1/ERK2, MAPK8/JNK1, MAPK14/p38), stimulating transcription through ELK1 and ATF2 phosphorylation.
• In the thymus, regulates CD4(+)/CD8(+) double positive thymocyte selection; in CD4 memory T-cells, links TCR signaling to production of IL2, IFNG, and IL4.
• Mediates dendritic cell survival by connecting TLR4 signaling to temporal BCL2 expression, and regulates differentiation and survival of osteoclasts.
• Phosphorylates CREB1 on Ser-133 in hippocampal neuron nuclei, contributing to long-term potentiation (LTP) and memory consolidation.
• Localizes to both the cytoplasm and nucleus.
• Expressed in brain, thymus, CD4 T-cells, testis, and epithelial ovarian cancer tissue.
• Belongs to the protein kinase superfamily, CaMK family; contains ATP-binding and calmodulin-binding domains; undergoes phosphorylation and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 304–473 of human CAMK4 (C-terminal region). Validate detection of full-length protein and potential isoforms in your specific sample system.
• For western blot, expect a band near 52 kDa; include appropriate positive controls (e.g., brain, thymus, or T-cell lysates) and molecular weight markers.
• In IHC-P, optimize antigen retrieval and antibody titration; cross-reactivity with mouse and rat tissues supports translational studies.
• In IF/ICC, note that CAMK4 shows both cytoplasmic and nuclear staining; fixation/permeabilization conditions may influence localization patterns.
• ELISA can be used for quantitative assays, but consider pairing with validated capture/detection antibodies for sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes by providing one-stop access to validated IVD raw materials, including high-quality antibodies, recombinant proteins, and ancillary reagents. For projects involving calcium signaling, neurobiology, or immune response, we offer tailored sourcing solutions and technical consulting to accelerate your development from concept to clinic. Contact us to explore bulk supply, custom antibody services, and rare target reagent sourcing.