Anti-CAMK2G Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q13555

Core Product Specifications and Parameters

Biological Background: CAMK2G Function and Localization

• CAMK2G (CAMK2G gene; aliases CAMK, CAMKG, MRD59, CAMK-II) encodes a calcium/calmodulin-dependent kinase type II subunit gamma, a member of the CAMK2 serine/threonine kinase family, with multiple isoforms generated by alternative splicing.
• The kinase becomes autonomously active after Ca2+/calmodulin binding and autophosphorylation, participating in key signal transduction pathways.
• In skeletal muscle, CAMK2G regulates sarcoplasmic reticulum Ca2+ transport: in slow-twitch muscle it controls SR Ca2+ uptake, while in fast-twitch muscle it modulates Ca2+ release via triadin phosphorylation.
  Related references: PMID:16690701
• Within the central nervous system, CAMK2G contributes to neurite formation and arborization, promoting dendritic spine and synapse formation and maintaining synaptic plasticity essential for learning and memory.
  Related references: PMID:30184290
• Stimulation by interferon-gamma induces CAMK2G-mediated phosphorylation of STAT1, activating the JAK-STAT signaling cascade (By similarity).
• Subcellularly, it localizes to the sarcoplasmic reticulum membrane, consistent with its role in intracellular calcium dynamics.
• Tissue specificity: predominantly expressed in skeletal muscle.
• The protein is classified under keywords such as ATP-binding, calmodulin-binding, kinase, transferase, serine/threonine-protein kinase, phosphoprotein; genetic variants are linked to intellectual disability (MRD59) and neurogenesis.

Experimental Guidance and Technical Tips

• The immunogen covers the regulatory domain (aa 300–410) of human CAMK2G; this region may confer specificity over other CAMK2 family members. Validate antibody specificity in your sample system using appropriate controls.
• For Western blotting, a band near 63 kDa (predicted molecular weight 62.6 kDa) is expected. Adjust blocking and antibody dilution conditions as needed.
• When performing immunohistochemistry (IHC-P) or immunofluorescence (IF-P), consider antigen retrieval protocols (e.g., heat-mediated citrate buffer) to unmask epitopes.
• The antibody has been reported to cross-react with mouse and rat CAMK2G; however, lot-to-lot consistency should be verified for each application.
• For ELISA, use as a capture or detection antibody after determining optimal coating concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This CAMK2G polyclonal antibody supports research in neuroscience and muscle physiology; CamelBio also offers validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to accelerate your immunoassay development. For rare targets or custom sourcing needs, our team ensures reliable, scalable supply from discovery through production.