Anti-CAMK2A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UQM7

Core Product Specifications and Parameters

Biological Background: CAMK2A Function and Localization

• CAMK2A (CaMKIIα) is a calcium/calmodulin-dependent serine/threonine-protein kinase that, upon autophosphorylation, becomes autonomously active, playing a central role in synaptic plasticity, neurotransmitter release, and long-term potentiation. Related references: PMID:14722083
• The kinase regulates dendritic spine morphogenesis and neuronal migration, essential for proper brain development and connectivity. Related references: PMID:28130356 PMID:29100089
• CAMK2A phosphorylates transcription factors such as FOXO3 to activate its transcriptional activity and ETS1 to modulate DNA binding, linking calcium signaling to gene expression. Related references: PMID:23805378
• In immune signaling, CAMK2A phosphorylates STAT1 in response to interferon stimulation (both IFN-gamma and IFN-beta), promoting the JAK-STAT pathway. Related references: PMID:11972023 PMID:35568036
• CAMK2A contributes to ferroptosis inhibition by phosphorylating PSAT1, which stabilizes GPX4 through hydroxylation. Related references: PMID:40281343
• Subcellularly, CAMK2A is enriched at the postsynaptic density of excitatory synapses, particularly in dendritic spines and dendrites, consistent with its role in synaptic signaling.
• Keyword context: The protein undergoes alternative splicing and is subject to palmitoylation, autophosphorylation, and ATP binding.
• As a member of the CAMK2 family, CAMK2A shares conserved kinase and calmodulin-binding domains but has unique roles in learning and memory.

Experimental Guidance and Technical Tips

• The antibody is raised against a peptide within amino acids 300-400 of human CAMK2A; consider sequence conservation across species and isoforms when interpreting results.
• For Western blot (WB), the predicted molecular weight is ~54 kDa; validate band specificity in relevant sample systems (e.g., brain tissue lysates) and account for possible post-translational modifications.
• Optimize primary antibody concentrations for immunofluorescence (IF/ICC) and ELISA based on your experimental setup; include appropriate positive and negative controls.
• Confirm cross-reactivity in mouse and rat models if using non-human samples, as indicated by the supplier's data.
• For ELISA, consider pairing with a detection antibody targeting a distinct epitope to avoid competition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for one-stop access to IVD raw materials, offering validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This CAMK2A antibody supports neuroscience and cell signaling research, and we can assist in scaling your project with tailored procurement solutions and technical support from concept to clinic.