Anti-CAMK2A Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q9UQM7

Core Product Specifications and Parameters

Biological Background: CAMK2A Function and Localization

• Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A) is a serine/threonine-protein kinase that becomes autonomously active upon Ca²⁺/calmodulin binding and autophosphorylation, playing a central role in synaptic plasticity, neurotransmitter release, and long-term potentiation (LTP).
  Related references: PMID:14722083
• CAMK2A is a key component of the NMDAR signaling complex at excitatory synapses, where it regulates AMPA receptor (AMPAR) trafficking and synaptic strength, thus modulating excitatory synaptic transmission (By similarity).
• Beyond synaptic functions, CAMK2A phosphorylates multiple substrates including FOXO3 to promote its transcriptional activity (PubMed:23805378) and ETS1 to reduce its DNA-binding affinity in response to calcium signaling (By similarity).
  Related references: PMID:23805378
• In the immune context, CAMK2A mediates interferon signaling by phosphorylating STAT1 upon IFN-gamma stimulation (PubMed:11972023) and IFN-beta stimulation (PubMed:35568036). It also phosphorylates PSAT1 to inhibit ferroptosis via GPX4 stabilization (PubMed:40281343).
  Related references: PMID:11972023, PMID:35568036, PMID:40281343
• Subcellularly, CAMK2A localizes to the postsynaptic density, dendritic spines, and dendrites, consistent with its role in synaptic function and neuronal morphology regulation.
• CAMK2A is subject to alternative splicing and post-translational modifications, including palmitoylation and autophosphorylation, which modulate its activity and localization (UniProt keywords).
• Mutations in CAMK2A are associated with intellectual disability and neurodevelopmental disorders, highlighting its essential role in brain development and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 201-300 of human CAMK2A, a sequence conserved in mouse and rat, supporting the observed cross-reactivity.
• For Western blotting, a band at approximately 54 kDa is expected; consider using neuronal tissue lysates (e.g., brain cortex) or cell lines expressing CAMK2A as positive controls.
• In immunofluorescence-paraffin (IF-P) experiments, target localization to dendritic spines and postsynaptic densities in brain sections; optimal fixation and permeabilization conditions should be validated.
• For ELISA applications, a synthetic peptide or recombinant protein spanning the immunogen region may serve as coating antigen; assay conditions require individual optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like anti-CAMK2A, for neuroscience and signal transduction research. As a one-stop sourcing partner for diagnostic manufacturers and laboratories, we provide custom antibody pairs, bulk reagents, and expert support to accelerate your development pipeline from concept to clinic.