Anti-CaMK2 delta/CAMK2 gamma Rabbit Monoclonal Antibody for WB, IP, ELISA - Q13557 / Q13555

Core Product Specifications and Parameters

Biological Background: CaMK2 delta/CAMK2 gamma Function and Localization

• CAMK2D encodes calcium/calmodulin-dependent protein kinase type II subunit delta (CaMKIIδ), a serine/threonine kinase belonging to the CaMK2 family. Alternative splicing yields tissue-specific isoforms (e.g., δ2, δ3, δ8, δ9 in heart; δ11 in skeletal muscle).
• In the nucleus, CaMKIIδ phosphorylates HDAC4, promoting its nuclear export and de-repression of MEF2 transcription factors, which drives expression of genes involved in cardiac hypertrophy and remodeling. Related references: PMID:17179159
• CaMKIIδ phosphorylates phospholamban (PLN), relieving its inhibition of SERCA2A and enhancing sarcoplasmic reticulum (SR) Ca²⁺ uptake; this is critical for frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. Related references: PMID:16690701
• The kinase localizes to the sarcolemma, sarcoplasmic reticulum membrane, and cell membrane, positioning it to rapidly modulate ion channels and Ca²⁺ handling proteins involved in excitation-contraction coupling.
• Predominantly expressed in cardiac muscle (isoforms δ2, δ3, δ8, δ9) and skeletal muscle (isoform δ11), reflecting its essential role in striated muscle physiology.
• CaMKIIδ directly phosphorylates multiple ion channels and transporters, including the L-type Ca²⁺ channel subunit CACNB2, the cardiac Na⁺ channel Nav1.5/SCN5A, and the K⁺ channel Kv4.3/KCND3, thereby influencing arrhythmogenesis in heart failure.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 200–300 of human CaMK2δ/γ (Q13554), a region conserved between both isoforms; the antibody is expected to recognize both delta and gamma subunits.
• For Western blot (WB), anticipate a primary band near 73 kDa; alternative splicing and post-translational modifications may produce additional bands—validate with relevant positive controls (e.g., cardiac tissue lysate).
• In immunoprecipitation (IP), optimize the antibody concentration and incubation conditions to ensure efficient capture of native CaMKII complexes.
• For ELISA detection, the immunogenic peptide can serve as a positive control; titrate the antibody to achieve an optimal dynamic range.
• Cross-reactivity with human, mouse, and rat makes this antibody suitable for cross-species studies; however, verify expression levels in your specific model system.
• Given the role of CaMKIIδ in cardiac signaling, cardiomyocyte lysates or heart tissue extracts are recommended as positive controls for initial assay validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of pre-validated IVD raw materials, including high-affinity monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. For projects targeting cardiac kinases and excitation-contraction coupling, we offer streamlined access to rare-target raw materials and provide end-to-end technical support—from concept to clinical application. Partner with CamelBio to accelerate your assay development and secure a reliable supply chain for critical reagents.