Anti-CAMK1D Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IU85

Core Product Specifications and Parameters

Biological Background: CAMK1D Function and Localization

• CAMK1D, also known as CaM kinase I delta or CKLiK, is a serine/threonine-protein kinase encoded by the CAMK1D gene and a member of the CaM kinase family.
• Operates in the calcium-triggered CaMKK-CaMK1 signaling cascade, activating CREB-dependent gene transcription, regulating granulocyte respiratory burst, and promoting hippocampal neuron dendritic growth.
• Subcellularly, CAMK1D localizes to both cytoplasm and nucleus.
• Widely expressed across tissues, with highest levels in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) and low to undetectable in monocytes and lymphocytes.
• May contribute to apoptosis in erythroleukemia cells and can phosphorylate transcription factor CREM isoform Beta.
• Features calmodulin-binding and ATP-binding domains, undergoes alternative splicing, and is regulated by allostery; implicated in inflammatory response and neurogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-180 amino acids of human CAMK1D, a region conserved across human, mouse, and rat, consistent with the reported cross-reactivity. For Western blot (WB), expect a band near 43 kDa. Optimize blocking and incubation steps for each application.
• For immunofluorescence (IF/ICC), choose fixation/permeabilization protocols that preserve nuclear and cytoplasmic epitopes; verify staining in a cell line with confirmed expression (e.g., HL-60 for granulocytic lineage).
• As a polyclonal antibody, lot-to-lot performance should be validated. Potential cross-reactivity with other CaMK kinases may occur; specificity can be confirmed using knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw-material sourcing, addressing the needs of diagnostic manufacturers and research institutes from concept to clinic. For targets like CAMK1D involved in calcium signaling and inflammation, we provide validated polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. Our capabilities also include rare target raw-material sourcing, ensuring you have reliable access to the critical components for your assay development.