Anti-CAMK1D Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IU85

Core Product Specifications and Parameters

Biological Background: CAMK1D Function and Localization

• CAMK1D, also known as CaM kinase I delta (CaMKI delta, CaMKID) or CKLiK, is a Ca²⁺/calmodulin-dependent serine/threonine kinase and a member of the CaMK1 family of protein kinases.
• Encoded by the CAMK1D gene, it functions in the calcium-triggered CaMKK-CaMK1 signaling cascade, where it activates CREB-dependent gene transcription upon calcium influx and regulates processes such as granulocyte function and hippocampal neuron dendritic growth.
• In neutrophils, CAMK1D is required for cytokine-induced proliferation and activation of the respiratory burst; it directly phosphorylates transcription factors CREB1 and CREM isoform Beta in vitro.
• The protein localizes to both the cytoplasm and the nucleus, consistent with its roles in signal transduction and transcriptional regulation.
• CAMK1D is widely expressed but shows highest expression in polymorphonuclear leukocytes (neutrophils and eosinophils), with little or no expression in monocytes and lymphocytes.
• Post-translational modifications include phosphorylation, ubiquitination (Ubl conjugation), and allosteric activation by Ca²⁺/calmodulin binding; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-180) of human CAMK1D, a domain highly conserved across species; the antibody is suitable for WB, IF/ICC, and ELISA.
• In western blot, the predicted molecular weight is 43 kDa, but additional bands may appear due to isoforms or post-translational modifications; consider optimizing blocking and dilution conditions.
• For immunofluorescence/immunocytochemistry, appropriate fixation and permeabilization are recommended to detect cytoplasmic and nuclear CAMK1D signals.
• Cross-reactivity with mouse and rat has been confirmed; however, it is advisable to validate performance in your specific sample system and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined, one-stop sourcing solution for IVD raw materials, specializing in high-quality antibodies and ancillary reagents. This anti-CAMK1D polyclonal antibody is part of our kinase biology portfolio, supporting immunodiagnostic development and research into calcium signaling and granulocyte function. We offer validated antibody pairs, bulk and custom antibody production, and rare-target sourcing to accelerate your project from concept to clinic.