Anti-Calumenin Monoclonal Antibody for WB, IHC-P, ELISA - O43852

Core Product Specifications and Parameters

Biological Background: Calumenin Function and Localization

• Calumenin (also known as Crocalbin and IEF SSP 9302) is a calcium-binding protein encoded by the CALU gene.
• It plays a regulatory role in vitamin K-dependent carboxylation, where it inhibits gamma-carboxylase GGCX activity on N-terminal glutamate residues.
• The protein binds up to 7 calcium ions with low affinity and is involved in secretory pathway calcium homeostasis.
• Calumenin localizes primarily to the endoplasmic reticulum membrane and Golgi apparatus, and is also found in the sarcoplasmic reticulum lumen, melanosomes, and can be secreted.
• It is ubiquitously expressed, with highest levels in heart, placenta, and skeletal muscle, lower in lung, kidney, and pancreas, and very low in brain and liver.
• Post-translational modifications include acetylation, alternative splicing, glycosylation, and phosphorylation, reflecting its dynamic regulation in cellular processes.

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to amino acids 200–300, a region likely exposed in the native protein; consider using it for detection of both membrane-bound and secreted forms.
• For Western blotting, a clear band at approximately 37 kDa is anticipated; ensure proper sample preparation to capture membrane-associated protein.
• For IHC-P, validate the antibody in tissues with known high expression, such as heart or placenta, and optimize antigen retrieval conditions.
• ELISA performance can be evaluated using recombinant Calumenin or cell lysates from positive control lines.
• Because Calumenin is secreted, analyzing cell culture supernatants may require concentration steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with verified reagents like this anti-Calumenin monoclonal antibody. Our portfolio supports calcium-binding protein and vitamin K metabolism studies, featuring validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to accelerate your assay development from concept to clinic.