Anti-CALU Rabbit Polyclonal Antibody for IF/ICC, ELISA - O43852

Core Product Specifications and Parameters

Biological Background: Calumenin Function and Localization

• Calumenin, also known as crocalbin or IEF SSP 9302, is a calcium-binding protein encoded by the CALU gene in humans.
• It is involved in the regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues; calumenin appears to inhibit the gamma-carboxylase GGCX.
• The protein binds 7 calcium ions with low affinity, suggesting a role in calcium-dependent signaling or folding processes within the secretory pathway.
• Subcellular localization includes the endoplasmic reticulum membrane, Golgi apparatus, and sarcoplasmic reticulum lumen; it is also secreted and found in melanosomes.
• Calumenin is ubiquitously expressed, with highest levels in heart, placenta, and skeletal muscle, lower levels in lung, kidney, and pancreas, and very low levels in brain and liver.
• Post-translational modifications include acetylation, phosphorylation, and glycosylation, and the protein undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-315 of human calumenin (NP_001210.1), which covers nearly the full length of the mature protein; this design may favor detection of native conformations in immunoassays.
• For immunofluorescence (IF/ICC), consider permeabilization protocols that allow access to the ER lumen or Golgi membranes where calumenin resides; co-localization with organelle markers is recommended.
• For ELISA applications, validate the antibody using recombinant calumenin protein and relevant biological matrices (e.g., cell lysates from human or rat tissues) to confirm sensitivity and specificity.
• Cross-reactivity with rat calumenin is listed, but users should verify reactivity in their specific model systems and consider sequence homology between species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a comprehensive, one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. The calumenin (CALU) target is relevant to studies of calcium homeostasis, vitamin K-dependent carboxylation, and secretory pathway biology; CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development. For rare targets or custom sourcing, our supply chain solutions bridge the gap between research needs and production-scale procurement.