Anti-Calretinin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P22676

Core Product Specifications and Parameters

Biological Background: Calretinin Function and Localization

• Calretinin is encoded by the CALB2 gene and is a 29 kDa calcium-binding protein (also known as 29 kDa calbindin). It serves as a critical intracellular calcium buffer and sensor.
• Via its EF-hand domains, it binds calcium ions and modulates downstream signaling pathways, maintaining neuronal calcium homeostasis (PubMed:2001709). Related references: PMID:2001709
• In the central nervous system, calretinin is predominantly expressed in discrete neuronal populations, where it regulates synaptic plasticity and neuronal excitability, contributing to cognitive functions such as learning and memory (By similarity).
• During neurodevelopment, calretinin plays a role in neuronal differentiation and maturation (By similarity).
• Subcellularly, the protein localizes to synapses, dendrites, and other cell projections, consistent with its role in neuronal signaling.
• Calretinin is subject to phosphorylation, suggesting dynamic regulation of its activity.
• It is a member of the calbindin family of calcium-binding proteins, characterized by multiple EF-hand repeats.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human calretinin protein (amino acids 1–271), implying that the antibody recognizes native and denatured forms, suitable for WB, IHC, IF, and ELISA.
• For Western blotting, a band corresponding to approximately 32 kDa (calculated molecular weight 31.5 kDa) is expected; consider using brain tissue lysates as a positive control.
• In immunohistochemistry and immunofluorescence, verify expression in known calretinin-positive structures (e.g., neuronal subsets in brain, mesothelial cells) and include appropriate negative controls.
• Given cross-reactivity with mouse and rat, this antibody can be employed in rodent model studies; however, always validate species-specific reactivity in the target tissue.
• Due to the neuronal enrichment, optimize antigen retrieval and detection protocols for paraffin-embedded brain sections (IHC-P).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply high-quality IVD raw materials like this anti-Calretinin monoclonal antibody. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—supporting your neuroscience and oncology diagnostic projects from concept to clinic.