Anti-Calretinin Polyclonal Antibody for WB, IHC, IF, ELISA - P22676

Core Product Specifications and Parameters

Biological Background: Calretinin Function and Localization

• Calretinin (CALB2), also known as 29 kDa calbindin, is a member of the EF-hand calcium-binding protein superfamily.
• It plays a key role in calcium homeostasis by buffering intracellular calcium levels and regulating calcium-dependent signal transduction. Related references: PMID:2001709
• In the brain, calretinin is predominantly expressed in specific neuronal subtypes where it modulates synaptic plasticity and neuronal excitability, contributing to learning and memory.
• During embryonic development, it promotes neuronal differentiation and maturation.
• The protein localizes to synapses and dendritic projections, supporting its function in neuronal communication.
• Calretinin is a phosphoprotein, indicating potential regulation by phosphorylation events.
• It is characterized as a metal-binding protein with multiple EF-hand repeats.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-271 of human Calretinin, suggesting the antibody recognizes epitopes across the entire protein sequence. This broad coverage may enhance detection in various applications.
• For Western blot analysis, consider using a positive control such as human brain lysate and expect a band at approximately 32 kDa.
• In immunohistochemistry (IHC) and immunofluorescence (IF), validate the antibody in the tissue of interest; paraffin-embedded sections may require antigen retrieval.
• The antibody is reported to cross-react with mouse and rat Calretinin; however, verify reactivity in your specific sample due to potential sequence variations.
• As a polyclonal antibody, lot-to-lot consistency should be evaluated; include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. Our expertise spans neuroscience and calcium signaling research tools like Calretinin antibodies, ensuring reliable supply for assay development. For specialized projects, CamelBio also offers tailored raw-material sourcing and technical consulting to accelerate your transition from concept to clinic.