Anti-Calreticulin Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P27797

Core Product Specifications and Parameters

Biological Background: Calreticulin Function and Localization

• Calreticulin (also known as CRP55, Calregulin, ERp60, HACBP, grp60) is a highly conserved calcium-binding chaperone predominantly located in the endoplasmic reticulum (ER) lumen.
• It promotes proper protein folding, oligomeric assembly, and quality control of newly synthesized glycoproteins through the calreticulin/calnexin cycle, interacting transiently with monoglucosylated substrates. Related references: PMID:7876246
• Calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor (NR3C1) and facilitates its nuclear export, linking it to transcriptional regulation. Related references: PMID:11149926
• Although primarily an ER luminal protein, calreticulin is also found in the cytosol, on the cell surface, secreted into the extracellular matrix, and within specialized vesicles such as cortical and cytolytic granules.
• In oocytes, calreticulin regulates calcium homeostasis and is exocytosed during cortical granule release, contributing to the block to polyspermy.
• The protein undergoes acetylation, hydroxylation, disulfide bond formation, and glycosylation and binds calcium and zinc, which are essential for its structural integrity and chaperone function.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 18–203 (N-terminal region) of human calreticulin, a conserved domain that likely accounts for the reported cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight is 47 kDa; use appropriate gel concentrations and confirm transfer efficiency with total protein staining.
• In immunohistochemistry and immunofluorescence, consider antigen retrieval and permeabilization steps due to the ER localization; subcellular localization may vary with cell type and physiological state.
• For ELISA applications, standard indirect ELISA protocols can be followed; titrate both primary antibody and antigen to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a full spectrum of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our calreticulin antibody supports research in ER stress, protein folding quality control, and calcium signaling. Whether you need rare target reagents or routine production-scale supplies, our integrated sourcing model helps diagnostic manufacturers and laboratories move from concept to clinic efficiently.