Anti-Calreticulin Rabbit Monoclonal Antibody (KO Validated) for WB, IHC-P, ELISA – P27797

Core Product Specifications and Parameters

Biological Background: Calreticulin Function and Localization

• Calreticulin, also known as CRP55, calregulin, ERp60, HACBP, and grp60, is encoded by the CALR gene.
• It functions as a calcium-binding chaperone that promotes the folding, oligomeric assembly, and quality control of newly synthesized glycoproteins in the endoplasmic reticulum via the calreticulin/calnexin cycle.
• As an ER resident lectin, it interacts transiently with nearly all monoglucosylated glycoproteins synthesized in the ER. Related references: PMID:7876246
• Calreticulin also mediates the nuclear export of the glucocorticoid receptor (NR3C1) by binding to its DNA-binding domain. Related references: PMID:11149926
• Subcellularly, it is primarily found in the ER lumen, but is also present in the cytosol, on the cell surface, and in the extracellular matrix, as well as in cytoplasmic vesicles such as cortical and cytolytic granules.
• The protein undergoes several post-translational modifications, including disulfide bond formation, acetylation, hydroxylation, and N-linked glycosylation, contributing to its structural and functional regulation.
• Calreticulin is implicated in maternal gene expression regulation and calcium homeostasis during oocyte maturation, and may participate in the block to polyspermy following fertilization.
• UniProt lists its molecular weight as 48,142 Da; the antibody recognizes a band at approximately 47 kDa by Western blot.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 18–203 of human Calreticulin (NP_004334.1), corresponding to the N-terminal domain; this region is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), a specific band at around 47 kDa is expected; use appropriate loading controls and validate in cell or tissue lysates known to express Calreticulin.
• KO validation confirms antibody specificity; consider using isogenic wild-type and Calreticulin knockout cell lines as negative controls in your experiments.
• In immunohistochemistry (paraffin sections, IHC-P), antigen retrieval may be required; optimize antibody dilution and detection conditions for your specific samples.
• For ELISA applications, the antibody can be used as a capture or detection reagent; pair with appropriate matched antibodies and validate in the relevant biological matrix.

CamelBio: Your One-Stop Sourcing Bridge

Calreticulin is a critical target in studies of ER stress, protein folding, and calcium signaling, with growing significance in cancer and autoimmune disease diagnostics. CamelBio provides one-stop access to IVD raw materials, including validated monoclonal antibodies like anti-Calreticulin, optimized antibody pairs, and ancillary reagents. Our supply capabilities encompass bulk quantities, rare target sourcing, and customizable formulations to support diagnostic manufacturers and research laboratories from concept to clinic.