Anti-Calponin-1 Rabbit Polyclonal Antibody for WB, ELISA - P51911

Core Product Specifications and Parameters

Biological Background: Calponin-1 Function and Localization

• Calponin-1, also known as basic calponin or calponin H1, is a smooth muscle-specific isoform encoded by the CNN1 gene. Alternative splicing generates additional variants.
• It is a thin filament-associated protein that binds actin, calmodulin, and tropomyosin, thereby regulating smooth muscle contraction by inhibiting actomyosin Mg-ATPase activity.
• Calponin-1 belongs to the calponin family of actin-binding proteins, which share a conserved calponin homology (CH) domain and are involved in cytoskeletal organization.
• Subcellularly, calponin-1 localizes to the contractile apparatus in smooth muscle cells, often associated with actin stress fibers.
• Post-translational modifications, particularly phosphorylation, modulate calponin-1’s interaction with actin and its inhibitory function, linking it to signaling pathways that control muscle tone.
• The protein is also identified in proteomic studies and features a 3D structure (available in databases), reflecting its importance in smooth muscle biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment covering amino acids 200–297 of human calponin-1 (NP_001290.2). Confirm that this region is relevant to your target isoform.
• This antibody is reported for use in Western blot (WB) and ELISA. For applications like immunohistochemistry (IHC) or immunoprecipitation (IP), validation in the relevant sample system is advised.
• Cross-reactivity is indicated for human, mouse, and rat samples; however, performance in other species or experimental conditions should be independently verified with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a one-stop portfolio of IVD raw materials, including validated antibody pairs and bulk ancillary reagents. For smooth muscle and cytoskeletal research, we offer optimized polyclonal and monoclonal antibodies, as well as sourcing of rare-target raw materials. Partner with CamelBio to streamline your assay development from concept to clinic with reliable procurement and technical guidance.