Anti-Calponin-1 Rabbit Monoclonal Antibody for WB, IHC, IF, ELISA - P51911

Core Product Specifications and Parameters

Biological Background: Calponin-1 Function and Localization

• Calponin-1, also known as basic calponin, smooth muscle calponin (SMCC), or Calponin H1, is encoded by the CNN1 gene.
• Predominantly expressed in smooth muscle and tissues containing significant amounts of smooth muscle.
• Functions as a thin filament-associated protein implicated in the regulation and modulation of smooth muscle contraction.
• Binds to actin, calmodulin, and tropomyosin; its interaction with actin inhibits the actomyosin Mg-ATPase activity.
• Subject to alternative splicing, generating multiple isoforms.
• Classified as a phosphoprotein, suggesting regulation by phosphorylation events.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 198-297 of human Calponin-1; verify that the epitope is present in the target isoform and species of interest.
• For Western blotting, a band near 33 kDa is expected in smooth muscle tissue lysates; include appropriate controls.
• For IHC-P, optimal detection may require antigen retrieval; test on paraffin-embedded smooth muscle sections.
• In IF-P, confirm localization to actin filaments in smooth muscle cells; counterstain with phalloidin or actin markers.
• For ELISA, consider pairing with a detection antibody targeting a non-overlapping epitope for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials, such as this Calponin-1 monoclonal antibody for smooth muscle contraction studies. Beyond this product, we supply validated antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to advance your IVD pipeline from concept to clinic. Contact us to discuss how we can support your next development project.