Anti-Calponin 1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P51911

Core Product Specifications and Parameters

Biological Background: Calponin-1 Function and Localization

• Calponin-1 (Basic calponin, Calponin H1) is encoded by the CNN1 gene and is a member of the calponin family of actin-binding proteins.
• It acts as a thin filament-associated regulatory protein in smooth muscle, capable of binding actin, calmodulin, and tropomyosin.
• By binding to actin, calponin-1 inhibits the actomyosin Mg-ATPase activity, thereby modulating smooth muscle contraction (By similarity).
• The protein is specifically expressed in smooth muscle and tissues rich in smooth muscle.
• Calponin-1 undergoes alternative splicing, yielding multiple transcripts.
• It is subject to phosphorylation, which may regulate its activity and interactions.
• Keywords: Actin-binding, Calmodulin-binding, Phosphoprotein, Repeat, and 3D-structure available.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 198–297, located in the central region of calponin-1. For applications requiring native conformation, consider that the epitope may be partially buried in filamentous assemblies.
• For Western blot analysis, the predicted molecular weight of calponin-1 is 33 kDa. Use appropriate positive controls (e.g., smooth muscle lysates from human, mouse, or rat) to confirm specificity.
• In IF-P and IHC-P, include suitable antigen retrieval steps and validate staining in smooth muscle-rich tissues. Blocking with serum from the same host as the secondary antibody is recommended.
• When performing ELISA, titrate the antibody and optimize coating conditions to achieve optimal signal-to-noise ratio. Cross-reactivity with mouse and rat calponin-1 may be advantageous for cross-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-Calponin 1 mAb for smooth muscle and cytoskeletal research. Our one-stop sourcing service streamlines procurement of antibody pairs, bulk ancillary reagents, and rare-target raw materials, accelerating your assay development from concept to clinic. Contact us to discuss customized supply solutions for your next project.