Anti-Calpain Small Subunit 1 (CAPNS1) Rabbit Monoclonal Antibody for WB, ELISA - P04632

Core Product Specifications and Parameters

Biological Background: Calpain Small Subunit 1 Function and Localization

• Calpain small subunit 1, also known as CAPNS1 or CAPN4, is the regulatory subunit of calpain, a calcium-activated neutral protease.
• Calpains are non-lysosomal thiol-proteases that mediate limited proteolysis of target proteins involved in cytoskeletal remodeling, cell migration, and signal transduction.
• This subunit is essential for calpain activity; deficiency leads to embryonic lethality, underscoring its critical role in development (By similarity).
• The protein localizes primarily to the cytoplasm and can associate with the cell membrane, consistent with its role in membrane-proximal signaling and cytoskeletal dynamics.
• Post-translational modifications include phosphorylation and acetylation, which may regulate its activity or interactions.
• Calpain small subunit 1 is a member of the calpain family and contains calcium-binding motifs, making it a key sensor of intracellular calcium signals.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 100 amino acids of human calpain small subunit 1; consider that this region may contain functional domains or interaction sites.
• For Western blot, the expected band is approximately 28 kDa in human, mouse, and rat lysates; validate in the relevant sample system.
• In ELISA applications, ensure the recombinant protein or peptide is properly coated and antibody binding is optimized under calcium-containing conditions if the epitope is conformation-sensitive.
• Since calpain small subunit 1 is a ubiquitous regulatory protein, appropriate positive and negative controls should be included, such as CAPNS1 knockout cell lines or tissues with known expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to calpain-related raw materials, including validated monoclonal antibodies like this calpain small subunit 1 antibody. Our portfolio covers antibody pairs, bulk ancillary reagents, and sourcing for rare targets across the protease and cytoskeletal research areas. Partner with us to streamline your reagent procurement from concept to clinic.