Anti-Calpain 2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17655

Core Product Specifications and Parameters

Biological Background: Calpain 2 Function and Localization

• Calpain-2 is the catalytic subunit of the calcium-activated neutral protease calpain 2, also known as M-calpain or millimolar-calpain, which requires millimolar calcium concentrations for activity.
• It functions as a calcium-regulated, non-lysosomal thiol-protease that mediates limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
• Known substrates include MYOC, proteolytically cleaved at Arg-226, and CPEB3, whose cleavage following neuronal stimulation relieves translational repression of target mRNAs. Related references: PMID:17650508
• Calpain-2 is ubiquitously expressed in human tissues.
• Subcellular localization includes the cytoplasm and association with the cell membrane.
• The protein undergoes post-translational modifications such as acetylation, phosphorylation, and ubiquitination, and features autocatalytic cleavage and isopeptide bond sites.

Experimental Guidance and Technical Tips

• For Western blot, use standard protocols and consider validating the antibody in your sample system; the predicted molecular weight is ~80 kDa.
• For immunohistochemistry (paraffin), optimize antigen retrieval conditions and validate in human, mouse, and rat tissues as cross-reactivity is indicated.
• For ELISA, titrate the antibody to determine the optimal working concentration for your assay format.
• The immunogen corresponds to the C-terminal region (aa 520-700) of human Calpain 2, a region conserved across species that may account for cross-reactivity with mouse and rat.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing solutions, including validated polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents. Our Calpain 2 antibody supports research in protease biology and calpain-mediated signaling pathways. We invite you to explore our full catalog and discuss custom sourcing needs to accelerate your diagnostic development.