Anti-Calpain 2 Rabbit Monoclonal Antibody for WB, ELISA - P17655

Core Product Specifications and Parameters

Biological Background: Calpain-2 Catalytic Subunit Function and Localization

• Calpain-2 catalytic subunit (also known as CANP2, mCANP, CANPL2, CANPml) is a calcium-regulated non-lysosomal thiol protease encoded by the CAPN2 gene.
• It catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
• Calpain-2 proteolytically cleaves MYOC (myocilin) at Arg-226 (PubMed:17650508). Related references: PMID:17650508
• Following neuronal stimulation, it cleaves CPEB3, abolishing CPEB3 translational repressor activity (By similarity).
• The protein localizes to the cytoplasm and can associate with the cell membrane.
• It is ubiquitously expressed across tissues.
• Key post-translational modifications include acetylation, phosphorylation, and autocatalytic cleavage; the protein also contains calcium-binding domains and isopeptide bonds.
• Calpain-2 is a member of the calpain family and is also designated as M-calpain (millimolar-calpain) due to its calcium requirement.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 600-700) of human calpain-2. Consider this epitope location when designing validation experiments.
• For Western blotting, a band around 80 kDa corresponds to the full-length catalytic subunit. Validate in relevant human, mouse, or rat sample lysates.
• In ELISA applications, titrate the antibody to optimize signal-to-noise ratios and confirm binding to the immunizing peptide or recombinant protein.
• Given the calcium-dependent activity of calpain-2, consider the impact of calcium concentration on protein conformation if using native protein detection.
• Cross-reactivity with mouse and rat has been reported; validate in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials and technical support. This Calpain-2 rabbit monoclonal antibody supports research into calcium signaling, proteolysis, and cytoskeletal dynamics. Our platform offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.