Anti-Calpain 1 Rabbit Polyclonal Antibody for WB, ELISA - P07384

Core Product Specifications and Parameters

Biological Background: Calpain 1 Function and Localization

• Calpain-1 is a calcium-regulated non-lysosomal thiol protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  Related references:
  PMID:19617626 | PMID:21531719 | PMID:2400579
• The enzyme proteolytically cleaves CTBP1 at Asn-375, Gly-387, and His-409, and activates caspase-7 (CASP7) to regulate apoptosis.
  Related references:
  PMID:23707407 | PMID:19617626
• Calpain-1 localizes to both the cytoplasm and the cell membrane, facilitating its role in membrane-proximal signaling events.
• It is ubiquitously expressed in human tissues, underscoring its housekeeping and regulatory functions.
• As a member of the peptidase C2 family, calpain-1 contains a cysteine active site and multiple EF-hand calcium-binding domains.
• Post-translational modifications include acetylation, autocatalytic cleavage, and phosphorylation, which modulate its activity.
• Mutations in the CAPN1 gene are linked to autosomal recessive hereditary spastic paraplegia (SPG76), highlighting its importance in neuronal health.

Experimental Guidance and Technical Tips

• For western blotting (WB), consider using the recombinant immunogen (amino acids 20–330) as a positive control to confirm band specificity.
• Calpain-1 activity is strictly calcium-dependent; include sufficient Ca²⁺ in lysis and assay buffers to maintain the protease in an active conformation.
• Validate antibody specificity by comparing signals in wild-type versus CAPN1 knockout or knockdown cell lysates.
• In ELISA applications, optimize coating and detection conditions using purified calpain-1 protein; the antibody can serve as either capture or detection reagent in a sandwich format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. For researchers targeting calpain-1 in calcium signaling, cytoskeletal dynamics, and neurodegeneration, we provide reliable access to rare-target raw materials and expert technical consultation. From concept to clinic, CamelBio streamlines your sourcing needs with quality-assured biologics and flexible supply solutions.