Anti-Calpain 1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P07384

Core Product Specifications and Parameters

Biological Background: Calpain 1 Function and Localization

• Calpain 1, encoded by CAPN1, is the catalytic subunit of the calcium-activated neutral protease calpain‑1, also known as mu‑calpain (muCANP).
• It functions as a calcium‑regulated non‑lysosomal thiol‑protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Related references: PMID:19617626, PMID:21531719, PMID:2400579
• Calpain 1 cleaves and activates caspase‑7 (CASP7), thereby linking its proteolytic activity to apoptotic pathways. Related references: PMID:19617626
• It proteolytically cleaves the transcriptional co‑repressor CTBP1 at specific residues (Asn‑375, Gly‑387, His‑409), implicating calpain‑1 in transcriptional regulation. Related references: PMID:23707407
• The protein localizes primarily to the cytoplasm and can associate with the cell membrane upon calcium activation.
• CAPN1 expression is ubiquitous across human tissues, underscoring its fundamental cellular role.
• Disease‑associated variants in CAPN1 cause hereditary spastic paraplegia (SPG76), highlighting the protein’s importance in neuronal maintenance.
• Post‑translational modifications, including autocatalytic cleavage, acetylation, phosphorylation, and ubiquitination, tightly regulate calpain 1 activity and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–200 of human Calpain 1; this region may be conserved in mouse and rat, which could explain the observed cross‑reactivity.
• For Western blot, consider reducing SDS‑PAGE conditions to detect the ~82 kDa Calpain‑1 large subunit; validate with appropriate positive controls.
• In IF/ICC experiments, permeabilization may be required to access intracellular calpain‑1; optimize fixation to preserve epitope recognition.
• For ELISA applications, coat plates with the immunogen peptide or recombinant protein and validate detection conditions in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. This anti‑Calpain 1 antibody targets a protease central to cytoskeletal remodeling and neurodegeneration research. Backed by bulk‑supply capabilities and rare‑target sourcing expertise, CamelBio supports your workflow from early development through clinical application.