Anti-Calpain 1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07384

Core Product Specifications and Parameters

Biological Background: Calpain-1 Function and Localization

• Calpain-1, encoded by the CAPN1 gene, is also known as calcium-activated neutral proteinase 1 (CANP 1), mu-calpain, or muCANP, and serves as the catalytic subunit of the micromolar-calpain protease.
• It functions as a calcium-regulated non-lysosomal thiol-protease, mediating limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Related references: PMID:19617626, PMID:21531719, PMID:2400579
• Calpain-1 specifically cleaves CTBP1 at asparagine-375, glycine-387, and histidine-409, and also cleaves and activates caspase-7 (CASP7). Related references: PMID:23707407, PMID:19617626
• Subcellularly, calpain-1 localizes to the cytoplasm and cell membrane.
• It is ubiquitously expressed across human tissues.
• Key features include autocatalytic cleavage, calcium-binding EF-hand domains, and susceptibility to acetylation and phosphorylation; disease-linked mutations in CAPN1 are associated with hereditary spastic paraplegia.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 82 kDa is expected; consider using appropriate positive and negative controls and validating in your sample system.
• For IHC-P, antigen retrieval methods such as heat-induced epitope retrieval may improve detection; optimize staining conditions for your specific tissue type and antibody dilution.
• For IF/ICC, permeabilization is required to detect intracellular calpain-1; fixation conditions should be carefully optimized.
• The antibody is predicted to recognize human, mouse, and rat calpain-1 based on sequence homology; cross-reactivity should be experimentally validated in the relevant model system.
• ELISA performance can be optimized by checkerboard titration of the antibody and antigen; use appropriate blocking and washing steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials, including validated antibody pairs and bulk reagents. For projects involving calpain-1—a key mediator of cytoskeletal remodeling and signal transduction—our team can source or produce optimized polyclonal antibodies and ancillary reagents to accelerate your assay development. Whether you need rare-target raw materials or cost-effective bulk supplies, CamelBio serves as your comprehensive procurement partner from concept to clinic.