Anti-Calnexin Polyclonal Antibody for WB, IF, IHC, ELISA - P27824

Core Product Specifications and Parameters

Biological Background: Calnexin Function and Localization

• Calnexin (IP90, p90) is a calcium-binding chaperone that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum, assisting in protein assembly and retention of unassembled subunits.
• It plays a critical role in ER quality control by retaining incorrectly folded proteins, preventing their exit to the Golgi.
• Calnexin is associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, possibly regulating thymocyte maturation.
• Subcellularly, it localizes to the endoplasmic reticulum membrane, with reported localization also to mitochondrion membrane and melanosome membrane.
• Post-translational modifications include acetylation, disulfide bond formation, phosphorylation, palmitoylation, and ubiquitination, which may regulate its function and localization.
• It contains a signal sequence, transmembrane domain, and lectin-like repeats, classifying it as a transmembrane lectin chaperone.
• The human Calnexin gene (CANX) encodes a protein of approximately 68 kDa (calculated molecular weight ~67.6 kDa).
• Calnexin is involved in host-virus interactions, playing roles in viral protein folding and immune evasion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 500–592). For Western blot, expect a band near 68 kDa; consider using reducing and non-reducing conditions to assess disulfide bond involvement.
• In immunofluorescence (IF/ICC), Calnexin typically exhibits an ER-like reticular staining pattern; include appropriate organelle markers for co-localization.
• For IHC-P, antigen retrieval may be necessary; validate with positive control tissues known to express Calnexin (e.g., pancreas, liver).
• The antibody shows cross-reactivity with mouse and rat; verify species-specific band sizes in your model system.
• For ELISA, the polyclonal nature may provide capture/detection flexibility; pair with monoclonal antibodies for sandwich ELISA format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and research institutions with comprehensive access to high-quality IVD raw materials. For Calnexin—a critical ER chaperone and target in protein folding disorders, cancer immunology, and infectious disease—we support your workflow with validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. Let CamelBio be your single partner from concept to clinic, combining supply reliability with technical expertise.