Anti-Calnexin Monoclonal Antibody for WB, ELISA - P27824

Core Product Specifications and Parameters

Biological Background: Calnexin Function and Localization

• Calnexin is a 67.6 kDa calcium-binding protein primarily located in the endoplasmic reticulum (ER), also known as IP90, p90, and major histocompatibility complex class I antigen-binding protein p88.
• It functions as a chaperone that specifically interacts with newly synthesized monoglucosylated glycoproteins, assisting in their proper folding and assembly within the ER.
• Calnexin plays a critical role in the ER quality control system by retaining incorrectly folded or unassembled protein subunits, thereby preventing their premature export to the Golgi.
• It may contribute to thymocyte maturation by associating with partial T-cell antigen receptor complexes, potentially acting as a signaling complex that regulates this process.
• Additional roles include participation in receptor-mediated endocytosis at the synapse, linking ER function to neuronal communication.
• Subcellular localization extends to the mitochondrion membrane and melanosome membrane, indicating broader cellular functions beyond the ER.
• Extensive post-translational modifications, including acetylation, phosphorylation, palmitoylation, and disulfide bond formation, regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 493–592 near the C-terminus of human calnexin; consider validating epitope accessibility in your target applications.
• For Western blot, use reducing conditions with appropriate protein loads; the predicted molecular weight is ~68 kDa, and you may observe additional bands due to post-translational modifications or isoforms.
• In ELISA, the peptide antigen can serve as a positive control; verify linearity and sensitivity using known concentrations of recombinant calnexin or cell lysates.
• Since this antibody is validated for human reactivity, validate performance in relevant human cell lines (e.g., HeLa, HEK293) or tissue samples before large-scale studies.
• Include appropriate negative controls (e.g., knockout lysates or peptide blocking) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined pathway to essential IVD raw materials, including this calnexin antibody for ER biology and protein folding studies. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials—all designed to accelerate your diagnostic development from concept to clinic. Partner with us to simplify your supply chain and focus on breakthrough outcomes.