Anti-Calmodulin (CALM3) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62158

Core Product Specifications and Parameters

Biological Background: Calmodulin Function and Localization

• The provided UniProt entry P62158 is currently inactive and does not contain detailed functional annotations. Calmodulin is a widely expressed calcium-binding protein that mediates numerous cellular processes, though specific details are unavailable from this record.
• Due to the inactive status of the UniProt record, no additional functional or localization data can be reliably extracted.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N‑terminal region (amino acids 1–149) of human CALM3. This region is highly conserved among calmodulin isoforms, so cross‑reactivity with other calmodulins (e.g., CALM1, CALM2) is possible. Validate in the relevant sample system if necessary.
• Predicted molecular weight is ~17 kDa; a band at this size should be observed in WB. Use appropriate positive and negative controls to confirm specificity.
• For IF/ICC, calmodulin localizes to both cytoplasm and nucleus; ensure permeabilization is optimized for antigen accessibility. Co‑staining with compartment‑specific markers may aid interpretation.
• In ELISA, consider using a blocking buffer without calcium chelators, as calmodulin conformation depends on Ca²⁺ binding. A competitive peptide blocking experiment may help confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For targets such as calmodulin, which plays a central role in calcium‑dependent signaling and gene expression, we offer one‑stop procurement and supply‑chain solutions. Our team also assists with rare‑target raw‑material sourcing and technical consulting, helping you streamline development from early‑stage research to clinical application.