Anti-Calmodulin 1/2/3 Rabbit Polyclonal Antibody for WB, ELISA - P0DP23/P0DP24/P0DP25

Core Product Specifications and Parameters

Biological Background: Calmodulin 1/2/3 Function and Localization

• Calmodulin is a ubiquitous calcium-binding protein that mediates calcium signal transduction by regulating numerous enzymes, ion channels, and aquaporins. Calcium-binding is required for its activation. Related references: PMID:16760425, PMID:23893133, PMID:26969752
• It stimulates protein kinases such as myosin light-chain kinases and CaMK2, and phosphatases, playing a central role in calcium-dependent cellular signaling.
• Calmodulin, together with CCP110 and centrin, regulates the centrosome cycle and progression through cytokinesis. Related references: PMID:16760425
• Mediates calcium-dependent inactivation of the L-type calcium channel CACNA1C. Related references: PMID:26969752
• Acts as a positive regulator of calcium-activated potassium channel KCNN2. Related references: PMID:27165696
• Forms a potassium channel complex with KCNQ1 and regulates its electrophysiological activity. Related references: PMID:25441029
• Functions as a sensor modulating endoplasmic reticulum contacts with other organelles via VMP1:ATP2A2. Related references: PMID:28890335
• Component of the SIFI complex required to terminate the mitochondrial stress response. Related references: PMID:38297121, PMID:40328314, PMID:40875847
• Subcellularly localized to the cytoplasm, cytoskeleton (spindle, spindle pole, microtubule organizing center, centrosome), and cell projections (cilium, flagellum).
• Required for Legionella pneumophila SidJ glutamylase activity and C. violaceum CopC/S. flexneri OspC3 arginine ADP-riboxanase activity.
• Key post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 70–149 of human calmodulin 1/2/3; it may recognize the denatured protein in Western blot under standard reducing conditions.
• Due to high sequence conservation, the antibody detects calmodulin from human, mouse, and rat; validate in the relevant sample system to confirm specificity.
• For Western blot, an expected band of approximately 16 kDa should be observed; consider using a high-resolution gel system for clear separation of this small protein.
• For ELISA, optimize coating antigen concentration and buffer conditions; the antibody is supplied as a polyclonal reagent, so lot-to-lot consistency should be verified in quantitative assays.
• This antibody is intended for research use only; it has not been validated for diagnostic or therapeutic applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to a comprehensive portfolio of IVD raw materials, technical services, and consulting—from concept to clinic. For calmodulin-related projects, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support sourcing of rare target raw materials. Our integrated supply chain ensures reliable delivery of high-quality reagents to accelerate your calmodulin research and assay development.