Anti-Calmodulin 1/2/3 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P0DP23 / P0DP24 / P0DP25

Core Product Specifications and Parameters

Biological Background: Calmodulin Function and Localization

• Calmodulin-1, encoded by the CALM1 gene, is a highly conserved calcium-binding messenger protein that transduces intracellular calcium signals.
• It mediates calcium signal transduction by controlling a large number of enzymes, ion channels, aquaporins, and other proteins through calcium-dependent binding. Related references: PMID:16760425 PMID:23893133 PMID:26969752
• The calcium-calmodulin complex stimulates various protein kinases, including myosin light-chain kinases and CaMK2, as well as phosphatases. Related references: PMID:16760425 PMID:23893133 PMID:26969752
• In conjunction with CCP110 and centrin, calmodulin participates in the genetic pathway regulating the centrosome cycle and cytokinesis. Related references: PMID:16760425
• Calmodulin regulates voltage-dependent L-type calcium channels (mediating calcium-dependent inactivation of CACNA1C) and positively modulates calcium-activated potassium channel KCNN2 activity. Related references: PMID:31454269 PMID:26969752 PMID:27165696
• It forms a potassium channel complex with KCNQ1, regulating its electrophysiological activity via calcium binding. Related references: PMID:25441029
• Acts as a sensor to modulate endoplasmic reticulum contacts with other organelles mediated by the VMP1:ATP2A2 complex. Related references: PMID:28890335
• Subcellularly, calmodulin localizes to the cytoplasm, cytoskeleton (spindle, spindle pole, microtubule organizing center, centrosome), and cell projections (cilium, flagellum).
• It is a component of the SIFI complex required to resolve mitochondrial stress responses and is also implicated in microbial infection processes (required for legionella SidJ glutamylase activity and bacterial ADP-riboxanase activity). Related references: PMID:38297121 PMID:40328314 PMID:40875847

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide spanning amino acids 70-149 of human Calmodulin (NP_008819.1). This region is highly conserved across Calmodulin isoforms and species, which may explain the reported cross-reactivity with mouse and rat.
• For Western blot, an expected band around 16 kDa should be observed in positive samples. Consider using calmodulin-enriched tissues or cell lines as positive controls.
• In immunofluorescence (IF-P), calmodulin may display a diffuse cytoplasmic staining with enrichment at the centrosome, spindle, or ciliary structures. Co-stain with appropriate organelle markers to validate localization.
• For ELISA, a direct or capture format can be explored, but optimal dilution should be determined empirically using the provided peptide or purified protein.
• Antibody performance in untested applications or species should be verified independently; consider including relevant positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with a complete sourcing solution for IVD raw materials. This anti-Calmodulin 1/2/3 antibody supports research into calcium signaling, channelopathies, and cytoskeletal dynamics. As your one-stop partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary supplies, and assistance in sourcing rare-target raw materials to bridge the gap from concept to clinical application.