Anti-Calmegin (CLGN) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - O14967

Core Product Specifications and Parameters

Biological Background: Calmegin Function and Localization

• Calmegin (CLGN) is a testis-specific endoplasmic reticulum chaperone that functions during spermatogenesis.
• It binds calcium ions and assists in folding client proteins critical for sperm adhesion onto the egg zona pellucida and subsequent penetration.
• Calmegin is required for sperm migration from the uterus into the oviduct and for normal male fertility.
• The protein localizes to the endoplasmic reticulum membrane.
• Tissue specificity: detected in testis at the protein level.
• The protein contains a signal peptide, transmembrane helix, and post-translational modifications including acetylation and phosphorylation; alternative splicing occurs.
• Calmegin has a calculated molecular weight of approximately 70 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 180–350 of human Calmegin, which may be accessible in both native and denatured conditions; consider this when designing experiments.
• For immunohistochemistry (IHC) or immunofluorescence (IF), validating antibody reactivity in testis tissue sections is recommended, as Calmegin is specifically expressed there.
• In western blotting (WB), a band around 70 kDa is expected; note that post-translational modifications or cleavage products could result in additional bands.
• This antibody has been reported to cross-react with mouse and rat Calmegin; verify detection in your species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For reproductive biology and fertility research, we can source rare-target reagents such as Calmegin antibodies and related proteins, streamlining your development from concept to clinic. Contact us to discuss your specific sourcing needs and technical requirements.