Anti-CALM2 Polyclonal Antibody for WB and ELISA - P0DP24

Core Product Specifications and Parameters

Biological Background: Calmodulin-2 Function and Localization

• Calmodulin-2 is a calcium-binding messenger that transduces calcium signals by regulating a broad array of proteins, including protein kinases (myosin light-chain kinases, CaMK2), phosphatases, ion channels, and aquaporins (PubMed:16760425, PubMed:26969752, PubMed:27165696).
  Related references: PMID:16760425 PMID:26969752 PMID:27165696
• Together with CCP110 and centrin, calmodulin-2 is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
• It mediates calcium-dependent inactivation of the L-type calcium channel CACNA1C.
• It positively regulates calcium-activated potassium channel activity of KCNN2.
• Required for the arginine ADP-riboxanase activity of bacterial effectors CopC (C.violaceum) and OspC3 (S.flexneri) during infection.
• Subcellular localization includes cytoplasm, cytoskeleton (spindle, spindle pole), and microtubule organizing center (centrosome).
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination; mutations in CALM2 are linked to Long QT syndrome (LQT15).

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (residues 1-149) of human CALM2, which is highly conserved across species. For western blotting, a specific band around 17 kDa is expected; consider using appropriate positive controls to confirm reactivity.
• The antibody is reported to cross-react with both human and mouse calmodulin-2, making it suitable for studies in these species. Validation in other species is recommended if needed.
• For ELISA applications, the recombinant immunogen can be used as a standard; optimize coating conditions and detection system for best sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For calmodulin-related research and diagnostic development, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your projects. Contact us to discuss how our supply solutions can support your next assay.