Anti-Caldesmon/CDM Rabbit Monoclonal Antibody for IHC-P, ELISA - Q05682

Core Product Specifications and Parameters

Biological Background: Caldesmon Function and Localization

• Caldesmon (CDM/HCAD/LCAD) is an actin- and myosin-binding protein encoded by the CALD1 gene; alternative splicing produces high-molecular-weight (smooth muscle) and low-molecular-weight (non-muscle) isoforms.
• It regulates actomyosin interactions by stimulating tropomyosin binding to actin, stabilizing actin filaments, and inhibiting actomyosin ATPase activity in muscle; inhibition is attenuated by calcium-calmodulin and potentiated by tropomyosin.
• Beyond contraction, caldesmon plays essential roles in cellular mitosis, receptor capping, and Schwann cell migration during peripheral nerve regeneration.
• Subcellularly, caldesmon localizes to the cytoplasm, particularly associated with actin stress fibers, myofibrils, and the cytoskeleton.
• Isoform 1 (high-molecular-weight) is predominantly expressed in smooth muscle, while isoforms 2-5 (low-molecular-weight) are widely distributed in non-muscle tissues and cells; not expressed in skeletal or cardiac muscle.
• UniProt keywords highlight calmodulin-binding, phosphoprotein, isopeptide bond, and Ubl conjugation, indicating regulatory post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen (aa 208–436) lies within a region conserved across caldesmon isoforms; validate detection of specific high- and low-molecular-weight forms in the relevant cell or tissue system.
• For IHC-P applications, use standard FFPE protocols; optimize antigen retrieval, blocking, and antibody titration for your sample type.
• In ELISA setups, the recombinant immunogen fragment can serve as a positive control to confirm assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated IVD raw materials, including monoclonal antibodies like this anti-Caldesmon clone. We support your development of smooth muscle and cytoskeleton-related assays with supply of optimized antibody pairs, bulk ancillary reagents, and sourcing for rare or custom targets. Whatever your project stage—from concept to clinic—contact CamelBio for reliable, one-stop procurement solutions.