Anti-CALD1 Rabbit Polyclonal Antibody for WB and ELISA - Q05682

Core Product Specifications and Parameters

Biological Background: Caldesmon Function and Localization

• The CALD1 gene encodes caldesmon, an actin- and myosin-binding protein that regulates actomyosin interactions in smooth muscle and non-muscle cells.
• Caldesmon acts as a bridge between myosin and actin filaments, stabilizes actin filament structure through tropomyosin binding, and inhibits actomyosin ATPase activity.
• Its inhibitory function on actomyosin ATPase is attenuated by calcium-calmodulin and potentiated by tropomyosin, highlighting a key calcium-dependent regulatory mechanism.
• Beyond muscle contraction, caldesmon participates in cellular mitosis, receptor capping, and Schwann cell migration during peripheral nerve regeneration (By similarity).
• High-molecular-weight isoform 1 is predominantly expressed in smooth muscles, while low-molecular-weight isoforms 2–5 are widely distributed in non-muscle tissues; caldesmon is not expressed in skeletal or cardiac muscle.
• Subcellularly, caldesmon localizes to the cytoplasm, cytoskeleton, stress fibers, and myofibrils.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin conjugation, reflecting its dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–240) of human CALD1, which may detect both high- and low-molecular-weight isoforms; verify isoform specificity in your sample system.
• For Western blotting, use appropriate protein extraction protocols and loading controls; caldesmon’s ~93 kDa size may require optimized gel conditions for clear resolution.
• When performing ELISA, consider pairing with a matched detection antibody for sandwich assay development and validate linearity and sensitivity using recombinant caldesmon standards.
• Since caldesmon is abundant in smooth muscle, smooth muscle tissue lysates serve as strong positive controls; skeletal and cardiac muscle are suitable negative controls due to lack of expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, technical services, and consulting from concept to clinic. For researchers focusing on smooth muscle pathology, cell motility, or cytoskeletal regulation, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing support for rare-target raw materials. Let CamelBio bridge your caldesmon research and diagnostic development needs with reliable supply solutions.