Anti-CALCR Polyclonal Antibody for WB, ELISA - P30988

Core Product Specifications and Parameters

Biological Background: Calcitonin Receptor Function and Localization

• Calcitonin receptor (CALCR) is a G-protein coupled receptor (GPCR) that mediates signaling in response to calcitonin, amylin, and calcitonin gene-related peptide (CGRP).
  Related references:
  PMID:35324283 PMID:38603770
• CALCR forms functional heteromeric complexes with receptor activity-modifying proteins (RAMP1, RAMP2, RAMP3) to generate amylin receptors (AMYR1-3), which exhibit distinct ligand selectivity and downstream signaling.
  Related references:
  PMID:35324283 PMID:38603770
• Ligand engagement triggers a conformational change, activating guanine nucleotide-binding proteins (G proteins) and stimulating the cAMP-dependent signaling pathway.
  Related references:
  PMID:35324283 PMID:38603770 PMID:7476993
• Alternative splicing and promoter usage yield both functional and non-functional receptor isoforms; the non-functional variant does not couple to G proteins, fails to activate adenylyl cyclase, and is not internalized upon ligand binding.
  Related references:
  PMID:7476993
• The receptor is a transmembrane glycoprotein containing disulfide bonds, characteristic of class B GPCRs, and localizes to the cell membrane.
• CALCR plays a central role in calcium homeostasis, bone metabolism, and energy balance through its actions in central and peripheral tissues.

Experimental Guidance and Technical Tips

• The immunogen (aa 25–148) lies within the extracellular N-terminal domain, which is exposed in native conformation; thus, the antibody may perform well in ELISA under non-denaturing conditions.
• For Western blot, a signal near 55 kDa is expected; consider possible glycosylation shifts that could increase the apparent molecular weight.
• Validate the antibody in your specific sample system, using appropriate positive (e.g., CALCR-overexpressing cells) and negative (e.g., knockdown or blocking peptide) controls.
• Co-immunoprecipitation or cross-linking studies with RAMP proteins may require optimization of buffer conditions to preserve receptor complexes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports calcitonin receptor research by offering validated antibody pairs, optimized polyclonal antibodies like this CALCR Rabbit pAb, and bulk ancillary reagents. As a one-stop IVD raw-material partner, we help diagnostic manufacturers and labs source rare-target raw materials, streamlining development from concept to clinic.