Anti-CALCOCO1 Rabbit Polyclonal Antibody for WB and ELISA - Q9P1Z2

Core Product Specifications and Parameters

Biological Background: CALCOCO1 Function and Localization

• CALCOCO1 (calcium-binding and coiled-coil domain-containing protein 1), also known as calphoglin or coiled-coil coactivator protein, is a multi-functional coactivator that interacts with aryl hydrocarbon and nuclear receptors through its N-terminal domain to regulate transcription.
• It serves as a secondary coactivator in the Wnt/CTNNB1 pathway by binding to CTNNB1 and enhancing LEF1-mediated transcriptional activation, with differential use of activation regions.
• Together with CCAR1, CALCOCO1 enhances GATA1- and MED1-driven transcription from the gamma-globin promoter during erythroid differentiation of K562 cells. Related references: PMID:24245781
• CALCOCO1 also forms part of the calphoglin complex, which links cellular phosphate and glucose metabolism with protein synthesis, degradation, calcium signaling, and cell growth by enhancing inorganic pyrophosphatase activity.
• Subcellular localization studies indicate CALCOCO1 is found in both the cytoplasm and nucleus, consistent with its roles in signaling and transcriptional regulation.
• The protein contains zinc-finger motifs and is phosphorylated, suggesting regulatory modifications; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody corresponds to amino acids 462-691 of human CALCOCO1 (NP_065949.1). The resulting antiserum is expected to recognize the full-length protein and may detect isoforms containing this region.
• For Western blotting, consider using whole-cell lysates from human or mouse cell lines known to express CALCOCO1 (e.g., K562 for the gamma-globin regulatory function). A band near 77 kDa is anticipated.
• ELISA applications may benefit from optimization of coating antigen concentration and antibody dilution to achieve desired sensitivity.
• Cross-reactivity with other species or isoforms should be validated in the relevant sample system, as the antibody has only been tested on human and mouse samples.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we specialize in providing diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This anti-CALCOCO1 antibody supports research into transcription regulation, Wnt signaling, and metabolic pathways. Whether you need bulk quantities, custom formulations, or rare-target sourcing, CamelBio streamlines your procurement from concept to clinic.