Anti-Calcineurin A Rabbit Monoclonal Antibody for WB, ELISA - Q08209

Core Product Specifications and Parameters

Biological Background: Calcineurin A Function and Localization

• Calcineurin A is the catalytic alpha subunit of the serine/threonine-protein phosphatase 2B (PP2B), encoded by the PPP3CA gene (also known as CALNA, CNA). It is a calcium/calmodulin-dependent enzyme critical for intracellular Ca2+ signal transduction.
• It dephosphorylates substrates harboring PxIxIT and/or LxVP docking motifs, enabling specific recognition in signaling cascades. Related references: PMID:17498738 PMID:17502104 PMID:22343722
• In immune signaling, it activates NFATC1 by dephosphorylation, promoting nuclear translocation and transcriptional activation, essential for T-cell antigen-specific proliferation. Related references: PMID:19154138 PMID:26248042
• Additionally, it dephosphorylates and inactivates transcription factor ELK1, modulates cofilin via SSH1, regulates mitochondrial fission through DNM1L dephosphorylation, and influences various other targets including DARPP32, CRTC2, TFEB, and KLHL3. Related references: PMID:15671020 PMID:18838687 PMID:19154138 PMID:30611118 PMID:33691586 PMID:30718414
• Subcellularly, it localizes to multiple compartments including cytoplasm, cell membrane, sarcolemma, Z line of myofibrils, and dendritic spines, reflecting its pleiotropic functions.
• It is expressed in keratinocytes and lymphoblasts, with demonstrated roles in skin barrier formation and immune responses. Related references: PMID:29043977 PMID:30254215
• Post-translationally modified by acetylation and nitration, and classified as an iron- and zinc-binding metallophosphoprotein; disease variants are linked to DEE91, craniosynostosis, and dwarfism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 422-521 within the catalytic domain of human Calcineurin A. This region is conserved across human, mouse, and rat, likely contributing to the observed cross-reactivity. Users performing WB should consider a starting dilution of 1:500-1:2000 and validate in relevant lysates. For ELISA, titrate the antibody to determine optimal working concentration.
• Since Calcineurin A is a ubiquitously expressed signaling molecule, it is advisable to include appropriate positive and negative controls, such as Jurkat T-cell lysates or tissues with known expression, to confirm specificity.
• The antibody is supplied in liquid form with ice bag shipping; aliquot and store at -20°C for long-term stability, avoiding freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop partner for IVD raw materials, offering bulk-quantity validated monoclonal antibodies like this anti-Calcineurin A clone, optimized antibody pairs, and ancillary reagents. Our portfolio accelerates diagnostic development in immunology, neuroscience, and oncology, with dedicated support for rare-target sourcing and technical consulting from concept to clinic. Contact us to secure consistent, high-performance reagents for your calcineurin signaling research and assay building.