Anti-CALCA Rabbit Polyclonal Antibody for IHC-P, ELISA - P06881

Core Product Specifications and Parameters

Biological Background: Calcitonin gene-related peptide 1 Function and Localization

• Calcitonin gene-related peptide 1 (alpha-CGRP, CGRP-I) is a peptide hormone encoded by the CALCA gene (CALC1), and is also known as CGRP-alpha.
• It functions as a potent vasodilator, mediating relaxation of coronary, cerebral, and systemic vasculature primarily through the CALCRL-RAMP1 receptor complex. Related references: PMID:1318039, PMID:33602864, PMID:9620797
• In the central nervous system, CGRP1 likely acts as a neurotransmitter or neuromodulator. Related references: PMID:3492492
• It elevates platelet cAMP levels, suggesting a role in platelet function. Related references: PMID:1318039
• CGRP1 can additionally bind and activate the CALCR-RAMP1 (AMYR1) receptor complex. Related references: PMID:38603770
• The protein is secreted and undergoes alternative splicing and post-translational modifications including amidation and disulfide bond formation.
• Tissue expression is notably found in the spinal cord, consistent with its neural activity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 26-128 of human CALCA (NP_001029125.1), covering the propeptide and mature CGRP region. This design may detect both precursor and processed forms.
• For IHC-P, consider antigen retrieval protocols optimized for spinal cord or vascular tissue sections, as CGRP expression is known in these tissues.
• For ELISA, validate the antibody in the appropriate biological matrix to confirm specificity and optimal dilution.
• Given the polyclonal nature and sequence similarity among calcitonin family peptides, cross-reactivity with related family members should be evaluated in the context of the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined supply chain for IVD raw materials, spanning from concept to clinic. For neurovascular and hormone research involving targets like CALCA/CGRP, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support your assay development and production needs. Whether you require rare target raw-material sourcing or technical consulting, our one-stop platform bridges the gap between discovery and diagnostic application.