Anti-Calbindin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P05937

Core Product Specifications and Parameters

Biological Background: Calbindin Function and Localization

• Calbindin (also known as Calbindin D28, D-28K, or CALB1) is a vitamin D-dependent calcium-binding protein belonging to the EF-hand superfamily.
• It functions as a cytosolic calcium buffer, maintaining intracellular Ca²⁺ homeostasis and protecting cells from calcium overload.
• Calbindin may also modulate signaling by stimulating membrane Ca²⁺-ATPase activity and 3',5'-cyclic nucleotide phosphodiesterase.
• The protein contains multiple EF-hand motifs that mediate high-affinity calcium binding.
• Post-translational modifications include acetylation, as indicated by UniProt keyword annotations.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity.
• The three-dimensional structure has been experimentally determined, offering insights into its calcium-binding mechanism.
• Calbindin is implicated in vitamin D-dependent calcium absorption and neurological processes, consistent with its tissue distribution.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human Calbindin, suggesting the antibody may recognize total protein; detectability of isoforms should be confirmed experimentally.
• For Western blot, a band around 30 kDa is expected; use appropriate molecular weight markers and loading controls.
• In IHC-P, optimize antigen retrieval (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and antibody dilution; include known positive and negative tissue controls.
• For ELISA, consider antibody pair compatibility and validate against recombinant protein or peptide standards.
• As a polyclonal antibody, lot-to-lot variability may occur; consistent lot testing is recommended for critical applications.
• Cross-reactivity with mouse and rat Calbindin has been demonstrated; verify reactivity in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw material partner, providing ready-to-use antibodies, bulk ancillary reagents, and expert technical support for diagnostic and research projects. This Calbindin rabbit polyclonal antibody is optimized for calcium signaling and neuroscience studies. We also source rare-target reagents and offer flexible procurement options to accelerate your development from concept to clinic.