Anti-Calbindin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P05937

Core Product Specifications and Parameters

Biological Background: Calbindin Function and Localization

• Calbindin (also known as Calbindin D28, D-28K, or Vitamin D-dependent calcium-binding protein, avian-type) is encoded by the CALB1 gene in humans.
• Functions primarily as a cytosolic calcium buffer, helping to regulate intracellular free calcium levels.
• May stimulate the activity of membrane Ca(2+)-ATPase and 3',5'-cyclic nucleotide phosphodiesterase, influencing calcium transport and cyclic nucleotide signaling.
• The protein contains EF-hand domains (calcium-binding motifs) and belongs to the calbindin family of calcium-binding proteins.
• Subject to alternative splicing and post-translational acetylation; keywords include Calcium, Metal-binding, Repeat, and Vitamin D.
• Expression is vitamin D-dependent, consistent with its role in calcium homeostasis.

Experimental Guidance and Technical Tips

• This polyclonal antibody is raised against full-length recombinant human Calbindin (aa 1-261). It may recognize multiple epitopes and offer robust detection in Western blot, immunohistochemistry (paraffin-embedded), and ELISA.
• For WB, the target band is expected at approximately 30kDa; consider using suitable protein ladders and transfer conditions.
• Since cross-reactivity is reported for human, mouse, and rat, validate performance in the specific species and sample type of interest.
• Optimize antibody dilution and blocking conditions empirically for each application; recommend using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibodies targeting calcium-binding proteins like calbindin. Our portfolio covers polyclonal antibodies, recombinant proteins, and ancillary reagents, streamlining your assay development from proof-of-concept to production.