Anti-Calbindin Rabbit Monoclonal Antibody for WB - P05937

Core Product Specifications and Parameters

Biological Background: Calbindin Function and Localization

• Calbindin (CALB1; D-28K) is a vitamin D-dependent calcium-binding protein belonging to the calbindin family, characterized by EF-hand repeats that bind calcium ions (Keyword: Calcium, Metal-binding, Repeat, Vitamin D).
• It functions as an intracellular calcium buffer, helping to maintain low resting cytosolic calcium concentrations and protecting cells from calcium overload (FUNCTION).
• Calbindin may stimulate the activity of membrane Ca(2+)-ATPase and 3',5'-cyclic nucleotide phosphodiesterase, linking calcium signaling to ATP-dependent ion transport and cyclic nucleotide metabolism (FUNCTION).
• The protein has a known three-dimensional structure (Keyword: 3D-structure), which has been extensively characterized.
• Alternative splicing of the CALB1 gene generates multiple isoforms (Keyword: Alternative splicing), potentially diversifying calbindin’s functional roles.
• Calbindin undergoes post-translational acetylation (Keyword: Acetylation), a modification that may regulate its activity or stability.
• It is a reference proteome component (Keyword: Reference proteome) and has been identified in multiple proteomics studies (Keyword: Proteomics identification), confirming its broad expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning the N-terminal region (amino acids 1–100) of human calbindin; this region is highly conserved across human, mouse, and rat, consistent with the broad cross-reactivity.
• For western blotting (WB), expect a band near 30 kDa; validate with appropriate positive and negative controls.
• In immunofluorescence on paraffin-embedded sections (IF-P), calbindin localization in tissues such as brain and kidney can be assessed; optimal dilutions and antigen retrieval methods should be determined experimentally.
• For ELISA applications, the antibody can be used as a capture or detection reagent; titrate to achieve desired sensitivity and specificity.
• Always confirm performance in your specific sample matrix and assay conditions, as recommended by regulatory guidelines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with a streamlined, one-stop platform for sourcing high-quality IVD raw materials. This Calbindin Rabbit mAb supports calcium signaling and neuroscience research, with applications in WB, IF-P, and ELISA. Complemented by our extensive portfolio of validated antibody pairs, bulk ancillary reagents, and expert technical consulting, CamelBio accelerates your assay development from early-stage research to clinical validation.