Anti-CADPS Polyclonal Antibody for WB, ELISA - Q9ULU8

Core Product Specifications and Parameters

Biological Background: CADPS Function and Localization

• CADPS encodes Calcium-dependent secretion activator 1 (CAPS-1), a calcium-binding protein essential for Ca²⁺-triggered exocytosis of dense-core vesicles (DCVs) containing neurotransmitters and neuropeptides.
• It acts upstream of membrane fusion, likely contributing to biogenesis or maintenance of mature secretory vesicles and regulating catecholamine loading into DCVs.
• CAPS-1 specifically mediates Ca²⁺-dependent exocytosis of large dense-core vesicles by binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and functioning at a prefusion step after ATP-dependent priming.
• Beyond DCVs, CADPS may also participate in small clear synaptic vesicle (SV) exocytosis, although its precise role in Ca²⁺ triggering remains to be fully elucidated.
• The protein localizes to the synapse, cytoplasmic vesicle membranes, and neuronal dense core vesicle membranes, consistent with its role in regulated secretion.
• Tissue expression is enriched in neural and endocrine secretory tissues, with highest levels in brain and pancreas and lower levels in heart; it is also detected in fetal cerebellum, cerebral cortex, medulla, and spinal cord.
• UniProt annotations highlight alternative splicing, calcium and lipid binding, phosphorylation, and involvement in protein transport, underscoring its multifunctional regulation in secretory pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (aa 1020-1100) of human CADPS; for Western blotting, expect a band near 153 kDa and consider using reducing SDS-PAGE with appropriate positive and negative controls.
• For ELISA applications, optimize coating antigen and antibody dilution using recombinant CADPS protein or peptide to ensure sensitivity and specificity.
• Reported cross-reactivity with mouse and rat makes this antibody suitable for rodent model studies; nonetheless, confirm species-specific reactivity in your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. For neuroscience and endocrine secretion research, we supply validated antibody pairs, optimized polyclonal antibodies like this anti-CADPS product, bulk ancillary reagents, and tailored sourcing for rare targets. Our integrated supply chain ensures reliable procurement to accelerate your assay development and biomarker discovery.