Anti-Cadherin 16 Monoclonal Antibody for WB, IF-P, ELISA - O75309

Core Product Specifications and Parameters

Biological Background: Cadherin-16 Function and Localization

• Cadherin-16 is a calcium-dependent cell adhesion protein that mediates homophilic cell-cell interactions, contributing to the sorting of heterogeneous cell types.
• It belongs to the cadherin superfamily, characterized by extracellular cadherin repeats, a single-pass transmembrane domain, and a conserved cytoplasmic tail.
• The protein is kidney-specific, suggesting a specialized role in renal cell adhesion and tissue organization.
• Subcellular localization is primarily at the cell membrane, consistent with its adhesive function.
• UniProt annotations highlight alternative splicing, glycosylation, phosphorylation, calcium-binding sites, and transmembrane domains, all typical of classical cadherins.
• The immunogen (aa 50-180) resides within the extracellular domain, potentially recognizing a functional adhesion-relevant epitope.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at ~90 kDa; use kidney tissue or cell lysates as positive controls.
• For immunofluorescence on formalin-fixed paraffin-embedded sections (IF-P), consider antigen retrieval and permeabilization to enhance antibody access to the extracellular epitope.
• Given its kidney specificity, validate antibody performance in human and mouse kidney samples to ensure specificity.
• Cross-reactivity with mouse enables translational studies; always include appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, with this anti-Cadherin-16 monoclonal antibody serving diagnostics developers targeting renal cell adhesion biomarkers. We offer validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and rare-target sourcing—helping you bridge from concept to clinical application efficiently.