Anti-Cadherin 16 (CDH16) Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - O75309

Core Product Specifications and Parameters

Biological Background: Cadherin-16 Function and Localization

• Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a member of the cadherin superfamily of calcium-dependent cell adhesion proteins.
• It mediates homophilic cell-cell adhesion, contributing to the sorting and organization of heterogeneous cell types within tissues.
• The protein is specifically expressed in the kidney, suggesting a role in renal epithelial organization and function.
• Cadherin-16 is a single-pass type I membrane protein localized at the cell membrane.
• It contains cadherin repeat domains characteristic of the family, and undergoes post-translational modifications including glycosylation and phosphorylation.
• Alternative splicing of the CDH16 gene results in multiple transcript variants.
• The protein binds calcium ions, which are essential for its adhesive function and structural integrity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 19-230), which may target the mature cadherin domain; consider this when designing experiments.
• For Western blot (WB), a band around ~90 kDa is expected; however, glycosylation may cause slight shifts, so validate in relevant lysates.
• In immunofluorescence (IF) and immunohistochemistry (IHC), optimize permeabilization to detect membrane-localized protein; kidney tissue sections are recommended.
• Cross-reactivity with mouse and rat is reported; validate antibody specificity in these species using appropriate controls.
• For ELISA, consider using the recombinant protein immunogen as a positive control to assess antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, connecting diagnostic developers and researchers with high-quality antibodies and ancillary reagents for every stage of development. For cadherin-16 research and kidney-specific applications, we offer access to validated polyclonal and monoclonal antibodies, including this Cadherin 16 rabbit pAb, along with customizable sourcing for rare targets. Our one-stop model covers everything from antigen design to final product integration, helping you accelerate your IVD project timelines.